GABA(A) Receptor-Associated Protein (GABARAP) (Tyr95) antibody

Antigen: GABA(A) Receptor-Associated Protein (GABARAP)

Synonyms: MM46, FLJ25768, MGC120154, MGC120155, mg:bb02b03, GABARAP, MGC128374

Binding Site: Tyr95

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650658

Additional Information

Alternative name: GABARAP

Gene ID: 11337

UniProt: O95166

Immunogen: This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86~115 amino acids from the C-terminal region of human GABARAP.

Isotype: Ig

Description: Other names: GABA(A) receptor-associated protein, MM46

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86~115 amino acids from the C-terminal region of human GABARAP.

Molecular Weight: 13787 DA

Comments:

Background: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

Application Details

Application Notes: The suggested dilution is: ELISA: ~~ 1:1,000 Western Blotting: ~~ 1:50~100 Immunohistochemistry: ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

(LEFT) Western blot analysis of GABARAP (arrow) using rabbit polyclonal Autophagy GABARAP Antibody (Y95) (ABIN650658). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GABARAP gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy GABARAP antibody (Y95)(ABIN650658), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Wang, Bedford, Brandon et al.: "GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton." in: Nature, Vol. 397, Issue 6714, pp. 69-72, 1999 (PubMed).