DNA-Damage Regulated Autophagy Modulator 1 (DRAM1) (N-Term) antibody

Antigen: DNA-Damage Regulated Autophagy Modulator 1 (DRAM1)

Synonyms: DRAM, FLJ11259, Dram, 1200002N14Rik, PRO180, TMEM77, WWFQ154, MGC54289, RP5-1180E21.1, DRAM1, dram, flj11259, MGC108229, MGC187403

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650661

Additional Information

Alternative name: DRAM

Gene ID: 55332

UniProt: Q8N682

Immunogen: This DRAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DRAM.

Isotype: Ig

Description: Other names: Damage-regulated autophagy modulator

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DRAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DRAM.

Molecular Weight: 26122 DA

Comments:

Background: This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Autophagy

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with *DRAM antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of anti-DRAM Antibody (N-term) (ABIN650661) in HepG2 cell line lysates (35ug/lane). DRAM (arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Green, Chipuk: "p53 and metabolism: Inside the TIGAR." in: Cell, Vol. 126, Issue 1, pp. 30-2, 2006 (PubMed).

Kerley-Hamilton, Pike, Hutchinson et al.: "The direct p53 target gene, FLJ11259/DRAM, is a member of a novel family of transmembrane proteins." in: Biochimica et biophysica acta, Vol. 1769, Issue 4, pp. 209-19, 2007 (PubMed).