KIAA0226 (KIAA0226) (Center) antibody

Antigen: KIAA0226 (KIAA0226)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650663

Additional Information

Alternative name: BARON

Gene ID: 9711

UniProt: Q92622

Immunogen: This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663~692 amino acids from the Central region of human BARON.

Isotype: Ig

Description: Other names: Uncharacterized protein KIAA0226, KIAA0226

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663~692 amino acids from the Central region of human BARON.

Molecular Weight: 108562 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of BARON Antibody (Center) (ABIN650663) in MCF-7 cell line lysates (35ug/lane). BARON (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

BARON Antibody (Center) (ABIN650663) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Venter, Adams, Myers et al.: "The sequence of the human genome." in: Science (New York, N.Y.), Vol. 291, Issue 5507, pp. 1304-51, 2001 (PubMed).

Muzny, Scherer, Kaul et al.: "The DNA sequence, annotation and analysis of human chromosome 3." in: Nature, Vol. 440, Issue 7088, pp. 1194-8, 2006 (PubMed).

Matsunaga, Saitoh, Tabata et al.: "Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages." in: Nature cell biology, Vol. 11, Issue 4, pp. 385-96, 2009 (PubMed).