Regulator of Chromosome Condensation 1 (RCC1) antibody

Antigen: Regulator of Chromosome Condensation 1 (RCC1)

Synonyms: CHC1, RCC1-I, Chc1, AI326872, 4931417M11Rik, zgc:65796, zgc:77114, wu:fj77f08, SNHG3-RCC1, RCC1, MGC148464, MGC187541, rcc1, chc1-a, BJ1, CG18640 (Bj1), RanGEF, inxs, sdt, DmelCG10480, CG10480, xrcc1, chc1-b, MGC115703, MGC132085

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN650668

Additional Information

Alternative name: RCC1

Gene ID: 1104

UniProt: P18754

Immunogen: This RCC1 antibody is generated from rabbits immunized with human RCC1 recombinant protein.

Isotype: Ig

Description: Other names: Regulator of chromosome condensation, Chromosome condensation protein 1, Cell cycle regulatory protein, RCC1, CHC1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RCC1 antibody is generated from rabbits immunized with human RCC1 recombinant protein.

Molecular Weight: 44969 DA

Comments:

Background: RCC1 promotes the exchange of Ran-bound GDP by GTP. It is involved in the regulation of onset of chromosome condensation in the S phase. RCC1 binds to chromatin. The RCC1/Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Patients with Raynaud disease produce antibodies that bind to RCC1.

Synonyms: CHC1, RCC1-I, Chc1, AI326872, 4931417M11Rik, zgc:65796, zgc:77114, wu:fj77f08, SNHG3-RCC1, RCC1, MGC148464, MGC187541, rcc1, chc1-a, BJ1, CG18640 (Bj1), RanGEF, inxs, sdt, DmelCG10480, CG10480, xrcc1, chc1-b, MGC115703, MGC132085

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Cycle, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RCC1 Antibody (ABIN650668) in 293, MDA-MB231 cell line lysates (35ug/lane). RCC1 (arrow) was detected using the purified Pab.

RCC1 Antibody (ABIN650668) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RCC1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Cushman, Stenoien, Moore: "The dynamic association of RCC1 with chromatin is modulated by Ran-dependent nuclear transport." in: Molecular biology of the cell, Vol. 15, Issue 1, pp. 245-55, 2003 (PubMed).

Li, Zheng: "Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells." in: Genes & development, Vol. 18, Issue 5, pp. 512-27, 2004 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).