|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Gfl-1 (N-Term) antibody
Western Blotting (WB), ELISA
|2 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This Gfl-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of C. Elegans Gfl-1.|
|Description||Other names: Hypothetical protein gfl-1, gfl-1,|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This Gfl-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of C. Elegans Gfl-1.|
|Molecular Weight||24949 DA|
Background: Gfl-1 encodes a C. elegans ortholog of human Glioma-amplified sequence-41 (GAS41), found in increased copy number in low-grade glioma. Loss of GLF-1, which is predicted to associate with chromatin, results in potent suppression of the RNA interference (RNAi) mechanism. GLF-1 is also similar to the transcription factors (yeast and human) AF-9 and human ENL, and thus may represent a novel class of transcription factors.
|Application Notes||The suggested dilution is: ELISA~~1:1,000 Western blot~~1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Transcription Factors, Signaling, Chromatin|
|Restrictions||For Research Use only|
|Gfl-1 Antibody (ABIN650678) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Gfl-1 antibody detected the Gfl-1 protein (arrow).|
Kim, Gabel, Kamath et al.: "Functional genomic analysis of RNA interference in C. elegans." in: Science (New York, N.Y.), Vol. 308, Issue 5725, pp. 1164-7, 2005 (PubMed).
Wang, Kennedy, Conte et al.: "Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants." in: Nature, Vol. 436, Issue 7050, pp. 593-7, 2005 (PubMed).