Gfl-1 (N-Term) antibody

Antigen: Gfl-1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650678

Additional Information

Gene ID: 187434

UniProt: Q21501

Immunogen: This Gfl-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of C. Elegans Gfl-1.

Isotype: Ig

Description: Other names: Hypothetical protein gfl-1, gfl-1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Gfl-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of C. Elegans Gfl-1.

Molecular Weight: 24949 DA

Comments:

Background: Gfl-1 encodes a C. elegans ortholog of human Glioma-amplified sequence-41 (GAS41), found in increased copy number in low-grade glioma. Loss of GLF-1, which is predicted to associate with chromatin, results in potent suppression of the RNA interference (RNAi) mechanism. GLF-1 is also similar to the transcription factors (yeast and human) AF-9 and human ENL, and thus may represent a novel class of transcription factors.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blot~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin

Restrictions: For Research Use only

Images

Gfl-1 Antibody (ABIN650678) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Gfl-1 antibody detected the Gfl-1 protein (arrow).

Publications

Product: Kim, Gabel, Kamath et al.: "Functional genomic analysis of RNA interference in C. elegans." in: Science (New York, N.Y.), Vol. 308, Issue 5725, pp. 1164-7, 2005 (PubMed).

Wang, Kennedy, Conte et al.: "Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants." in: Nature, Vol. 436, Issue 7050, pp. 593-7, 2005 (PubMed).