Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1) (N-Term, Ser10) antibody

Antigen: Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1)

Synonyms: RDL, Gm185, MGC100232, MGC102277, MGC95290, MGC77374, zgc:77374, LEO1, DKFZp459B2211

Binding Site: N-Term, Ser10

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650686

Additional Information

Alternative name: LEO1

Gene ID: 123169

UniProt: Q8WVC0, NP_620147.1

Immunogen: This Leo1-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids amino acids from the N-terminal region of human PMAT-Y198.

Isotype: Ig

Description: Other names: LEO1,RDL

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Leo1-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids amino acids from the N-terminal region of human PMAT-Y198.

Molecular Weight: 75404 DA

Comments:

Background: LEO1, parafibromin (CDC73, MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Leo1-pS10 (ABIN650686) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Leo1 antibody detected the Leo1 protein (arrow).

Leo1-S10 (ABIN650686) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane). This demonstrates the Leo1 antibody detected the Leo1 protein (arrow).

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Rozenblatt-Rosen, Hughes, Nannepaga et al.: "The parafibromin tumor suppressor protein is part of a human Paf1 complex." in: Molecular and cellular biology, Vol. 25, Issue 2, pp. 612-20, 2005 (PubMed).

Zhao, Tong, Zhang: "Expression of the Leo1-like domain of replicative senescence down-regulated Leo1-like (RDL) protein promotes senescence of 2BS fibroblasts." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 6, pp. 521-32, 2005 (PubMed).

Zhu, Zheng, Pham et al.: "Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation." in: Molecular cell, Vol. 20, Issue 4, pp. 601-11, 2005 (PubMed).