Plexin A2 (Plxna2) antibody

Antigen: Plexin A2 (Plxna2)

Synonyms: OCT, Plxn2, AA589422, AW457381, mKIAA0463, 2810428A13Rik, PLXN2, FLJ11751, FLJ30634, KIAA0463, oct, plxn2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650695

Additional Information

Alternative name: OCT

Gene ID: 5460

UniProt: Q01860

Immunogen: This OCT antibody is generated from rabbits immunized with a His fusion protein from human OCT.

Isotype: Ig

Description: Other names: POU domain class 5 transcription factor 1, POU5F1, Octamer-binding transcription factor 3, OCT-3, OTF3, OCT-4

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This OCT antibody is generated from rabbits immunized with a His fusion protein from human OCT.

Molecular Weight: 38562 DA

Comments:

Background: Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of OCT Antibody (ABIN650695) in A375 cell line lysates (35ug/lane). OCT (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with OCT Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

OCT Antibody (ABIN650695) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).

Crouau-Roy, Amadou, Bouissou et al.: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Looijenga, Stoop, de Leeuw et al.: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).