Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A) (N-Term) antibody

Antigen: Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A)

Synonyms: VIa, Semaq, AI851735, Sema6A-1, 9330158E07, A730020P05Rik, VIA, SEMA, HT018, SEMAQ, SEMA6A1, KIAA1368, sema6a, fj46c12, wu:fj46c12, SEMA6A

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650714

Additional Information

Alternative name: SEMA6A

Gene ID: 57556

UniProt: Q9H2E6, NP_065847.1

Immunogen: This SEMA6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human SEMA6A.

Isotype: Ig

Description: Other names: Semaphorin-6A,SEMA6A,SEMAQ, KIAA1368

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SEMA6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human SEMA6A.

Molecular Weight: 114369 DA

Comments:

Background: SEMA6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. However, the length of the cytoplasmic tail, which includes an EVL-binding site in SEMA6A and an Src-binding site in SEMA6B, suggests that these semaphorins may also function as receptors. SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. SEMA6A directly links the Ena/VASP and the semaphorin protein families since the SEMA6A protein is capable of selective binding to the protein EVL (Ena/VASP-like protein).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

SEMA6A Antibody (N-term) (ABIN650714) western blot analysis in A2058 cell line lysates (15ug/lane).This demonstrates the SEMA6A antibody detected the SEMA6A protein (arrow).

Publications

Product: Landers, Melki, Meininger et al.: "Reduced expression of the Kinesin-Associated Protein 3 (KIFAP3) gene increases survival in sporadic amyotrophic lateral sclerosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 22, pp. 9004-9, 2009 (PubMed).