Profilin 1 (PFN1) antibody

Antigen: Profilin 1 (PFN1)

Synonyms: Pfn, PFN1, profilin, MGC130883, Profilin1, XProfilin1, sb:cb813, wu:fa91a03, F6F22.21, F6F22_21, PRF1, PROFILIN 1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650742

Additional Information

Alternative name: PFN1

Gene ID: 5216

UniProt: NP_005013.1, P07737

Immunogen: This PFN1 Antibody is generated from rabbits immunized with a recombinant protein of human PFN1.

Isotype: Ig

Description: Other names: Profilin-1,PFN1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PFN1 Antibody is generated from rabbits immunized with a recombinant protein of human PFN1.

Molecular Weight: 15054 DA

Comments:

Background: The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Restrictions: For Research Use only

Images

PFN1 Antibody (ABIN650742) western blot analysis in MDA-MB435,Jurkat,Hela cell line lysates (15ug/lane).This demonstrates the PFN1 antibody detected the PFN1 protein (arrow).

PFN1 Antibody (ABIN650742) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Ampe, Markey, Lindberg et al.: "The primary structure of human platelet profilin: reinvestigation of the calf spleen profilin sequence." in: FEBS letters, Vol. 228, Issue 1, pp. 17-21, 1988 (PubMed).

Mellon, Frank, Fang: "Mast cell alpha-chymase reduces IgE recognition of birch pollen profilin by cleaving antibody-binding epitopes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 1, pp. 290-7, 2001 (PubMed).

Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Richer, Stewart, Tomaszewski et al.: "NMR investigation of the binding between human profilin I and inositol 1,4,5-triphosphate, the soluble headgroup of phosphatidylinositol 4,5-bisphosphate." in: Biochemistry, Vol. 47, Issue 51, pp. 13455-62, 2008 (PubMed).