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Heat Shock 70kDa Protein 8 (HSPA8) (N-Term), (AA 82-110) antibody

Details for Product No. ABIN650754
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Antigen
Synonyms hsc54, hsc70, hsc71, hsp71, hsp73, hspa10, lap1, nip71, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, NIP71, Hsc70, 2410008N15Rik, Hsc71, Hsc73, Hsp73, Hspa10, wu:fb01g06, wu:fi48b06
Epitope
N-Term, AA 82-110
(18), (10), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (60), (54), (24), (23), (18), (17), (9), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(100), (42), (13), (10)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(151), (73), (64), (55), (29), (28), (23), (19), (10), (10), (2), (2), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN650754
238.50 $
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Immunogen This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 AA from the N-terminal region of human HSPA8.
Clone RB17655
Isotype Ig
Specificity This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89~118 amino acids from the N-terminal region of human HSPA8.
Predicted Reactivity Cow (Bovine),Chicken,Hamster,Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HSPA8
Background HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
Synonyms: Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8,
Synonyms: HSPA10,
Synonyms: HSP73,
Synonyms: HSC70, name: HSPA8,
Molecular Weight 70898 DA
Gene ID 3312
UniProt P11142
Research Area Cell Signaling, Stem Cells, Cell Structure
Application Notes WB = 1:1000, IHC = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term), (AA 82-110) antibody Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term), (AA 82-110) antibody (2) Western blot analysis of HSPA8 Antibody (N-term) (ABIN650754) in Hela cell line lysates (35 µg/lane). HSPA8 (arrow) was detected using the purified polyclonal antibody.
anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term), (AA 82-110) antibody (3) HSPA8 Antibody (N-term) (ABIN650754) flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Egerton, Moritz, Druker et al.: "Identification of the 70kD heat shock cognate protein (Hsc70) and alpha-actinin-1 as novel phosphotyrosine-containing proteins in T lymphocytes." in: Biochemical and biophysical research communications, Vol. 224, Issue 3, pp. 666-74, 1996 (PubMed).

Validation Images
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