Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5) (Center) antibody

Antigen: Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide) (ITGA5)

Synonyms: CD11C, SLEB6, Cr4, N418, Cd11c, AI449405, ITGAX, ITGA5, CD11c

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650756

Additional Information

Alternative name: CD49e

Gene ID: 3678

UniProt: P08648

Immunogen: This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 616~646 amino acids from the Center region of human CD49e.

Isotype: Ig

Description: Other names: Integrin alpha-5, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49 antigen-like family member E, CD_antigen=CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CD49e antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 616~646 amino acids from the Center region of human CD49e.

Molecular Weight: 114536 DA

Comments:

Background: CD49e belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein is the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells

Restrictions: For Research Use only

Images

Western blot analysis of CD49e Antibody (Center) (ABIN650756) in MDA-MB231 cell line lysates (35ug/lane). CD49e (arrow) was detected using the purified Pab.

CD49e Antibody (Center) (ABIN650756) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Schornberg, Shoemaker, Dube et al.: "Alpha5beta1-integrin controls ebolavirus entry by regulating endosomal cathepsins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 19, pp. 8003-8, 2009 (PubMed).

Okazaki, Ni, Ayeni et al.: "alpha5beta1 Integrin blockade inhibits lymphangiogenesis in airway inflammation." in: The American journal of pathology, Vol. 174, Issue 6, pp. 2378-87, 2009 (PubMed).

Boehmler, Drost, Jaggy et al.: "The CysLT1 ligand leukotriene D4 supports alpha4beta1- and alpha5beta1-mediated adhesion and proliferation of CD34+ hematopoietic progenitor cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 6789-98, 2009 (PubMed).