Eukaryotic Translation Initiation Factor 6 (EIF6) (C-Term) antibody

Antigen: Eukaryotic Translation Initiation Factor 6 (EIF6)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650760

Additional Information

Alternative name: ITGB4BP

Gene ID: 3692

UniProt: P56537, NP_002203

Immunogen: This ITGB4BP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human ITGB4BP.

Isotype: Ig

Description: Other names: EIF6,ITGB4BP, EIF3A,Eukaryotic translation initiation factor 6

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ITGB4BP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human ITGB4BP.

Molecular Weight: 26599 DA

Comments:

Background: Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Signaling, Cell Structure

Restrictions: For Research Use only

Images

ITGB4BP Antibody (C-term) (ABIN650760) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ITGB4BP antibody detected the ITGB4BP protein (arrow).

ITGB4BP Antibody (C-term) (ABIN650760) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITGB4BP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Chendrimada, Finn, Ji et al.: "MicroRNA silencing through RISC recruitment of eIF6." in: Nature, Vol. 447, Issue 7146, pp. 823-8, 2007 (PubMed).

Tang, Niu, Xu et al.: "Alteration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation." in: World journal of gastroenterology : WJG, Vol. 13, Issue 20, pp. 2791-7, 2007 (PubMed).

Flavin, Smyth, Finn et al.: "Altered eIF6 and Dicer expression is associated with clinicopathological features in ovarian serous carcinoma patients." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 6, pp. 676-84, 2008 (PubMed).

Gudbjartsson, Walters, Thorleifsson et al.: "Many sequence variants affecting diversity of adult human height." in: Nature genetics, Vol. 40, Issue 5, pp. 609-15, 2008 (PubMed).