Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (pSer32, pSer36) antibody
| Antigen |
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| Synonyms |
IKBA, MAD-3, NFKBI |
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Binding Site
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pTyr42 (28), pSer32,pSer36 (21), pTyr305 (19), pSer32, pSer36 (12), C-Term (8), N-Term (6), pSer36,pSer32 (5), AA 32-291 (4), Ser32, Ser36 (3), AA 19-49 (2), AA 34-48 (2), Pser32,pSer36 (2), Ser32 (2), Ser32,Ser36 (2), Tyr181 (2), Tyr42 (2), AA 130-143 (1), AA 18-48 (1), AA 20-50 (1), AA 32-36 (1), AA 32-47 (1), Internal Region (1), Ser23 (1), Sumoylation Site (1), pSer32 (1), pSer320 (1), pSer36 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (219), Mouse (Murine) (137), Rat (Rattus) (110), Cow (Bovine) (59), Pig (Porcine) (59), Sheep (Ovine) (54), Dog (Canine) (22), Chicken (20), Cat (Feline) (1), Hamster (1), Rabbit (1)
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Conjugate
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Biotin (11), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
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Application
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Western Blotting (WB) (134), Immunofluorescence (IF) (85), ELISA (77), Immunohistochemistry (IHC) (63), Immunoprecipitation (IP) (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (9), Flow Cytometry (FACS) (5), Dot Blot (DB) (4), Immunoelectron Microscopy (IEM) (4), Gel Shift (GS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
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| Catalog no. |
ABIN650807 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
280.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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NFKBIA
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Gene ID
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4792
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UniProt
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P25963
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Immunogen
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This Phospho-NFKBIA-S32/36 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 19~49 amino acids surrounding S32/36 of human NFKBIA.
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Isotype
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Ig
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Description
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Other names: NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBI, MAD3, IKBA, NFKBIA
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Characteristics
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Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Specificity
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This Phospho-NFKBIA-S32/36 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 19~49 amino acids surrounding S32/36 of human NFKBIA.
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Molecular Weight
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35609 DA
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Comments
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Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
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Application Notes
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The suggested dilution is: ELISA ~~ 1:1,000 Dot blot ~~ 1:500
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Concentration
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0.25 mg/ml
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Purification
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Purified
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Buffer
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Purified polyclonal antibody supplied in PBS with 0.18% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Neurology
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Restrictions
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For Research Use only
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Product
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Shimada, Kawai, Takeda et al.: "IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases." in: International immunology, Vol. 11, Issue 8, pp. 1357-62, 1999 (PubMed).
Gil, AlcamÃ, Esteban: "Activation of NF-kappa B by the dsRNA-dependent protein kinase, PKR involves the I kappa B kinase complex." in: Oncogene, Vol. 19, Issue 11, pp. 1369-78, 2000 (PubMed).
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Alternatives for antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", type "Antibodies"
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Hosts
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Rabbit (196), Mouse (26), Sheep (4), Goat (1)
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Reactivities
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Human (219), Mouse (Murine) (137), Rat (Rattus) (110), Cow (Bovine) (59), Pig (Porcine) (59), Sheep (Ovine) (54), Dog (Canine) (22), Chicken (20), Cat (Feline) (1), Hamster (1), Rabbit (1)
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Applications
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Western Blotting (WB) (134), Immunofluorescence (IF) (85), ELISA (77), Immunohistochemistry (IHC) (63), Immunoprecipitation (IP) (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (9), Flow Cytometry (FACS) (5), Dot Blot (DB) (4), Immunoelectron Microscopy (IEM) (4), Gel Shift (GS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
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Conjugates
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Biotin (11), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
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Epitopes
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pTyr42 (28), pSer32,pSer36 (21), pTyr305 (19), pSer32, pSer36 (12), C-Term (8), N-Term (6), pSer36,pSer32 (5), AA 32-291 (4), Ser32, Ser36 (3), AA 19-49 (2), AA 34-48 (2), Pser32,pSer36 (2), Ser32 (2), Ser32,Ser36 (2), Tyr181 (2), Tyr42 (2), AA 130-143 (1), AA 18-48 (1), AA 20-50 (1), AA 32-36 (1), AA 32-47 (1), Internal Region (1), Ser23 (1), Sumoylation Site (1), pSer32 (1), pSer320 (1), pSer36 (1)
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Alternatives 
