APG8c (MAP1LC3C) (pTyr105) antibody

Antigen: APG8c (MAP1LC3C)

Binding Site: pTyr105

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650831

Additional Information

Gene ID: 440738

UniProt: Q9BXW4

Immunogen: This Phospho-APG8c (MAP1LC3C)-Y105 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 91~121 amino acids surrounding Y105 of human APG8c (MAP1LC3C).

Isotype: Ig

Description: Other names: Autophagy-related ubiquitin-like modifier LC3 C, Autophagy-related protein LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, MAP1A/MAP1B LC3 C, Microtubule-associated protein 1 light chain 3 gamma, Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Phospho-APG8c (MAP1LC3C)-Y105 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 91~121 amino acids surrounding Y105 of human APG8c (MAP1LC3C).

Molecular Weight: 16852 DA

Comments:

Background: APG8c is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. APG8c is 1 of 3 orthologs of the yeast autophagosome protein Atg8.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot Blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Phospho-specific antibodies

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Phospho-APG8c (MAP1LC3C)-Y105 Phospho-specific Pab (ABIN650831) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Publications

Product: He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).