Hydroxy-HIF1A (Pro564) antibody

Antigen: Hydroxy-HIF1A

Binding Site: Pro564

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650844

Additional Information

Gene ID: 3091

UniProt: Q16665

Immunogen: This Hydroxy-HIF1A-P564 antibody is generated from rabbits immunized with a KLH conjugated synthetic hydroxypeptide between 547~577 amino acids surrounding P564 of human HIF1A.

Isotype: Ig

Description: Other names: Class E basic helix-loop-helix protein 78, bHLHe78, PAS domain-containing protein 8, Basic-helix-loop-helix-PAS protein MOP1, Member of PAS protein 1, ARNT-interacting protein, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha,PASD8, MOP1, BHLHE78, HIF1A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Hydroxy-HIF1A-P564 antibody is generated from rabbits immunized with a KLH conjugated synthetic hydroxypeptide between 547~577 amino acids surrounding P564 of human HIF1A.

Molecular Weight: 92670 DA

Comments:

Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blot~~ 1:500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step hydroxyspecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Hydroxy-HIF1A (HyP564) Antibody Pab (ABIN650844) on nitrocellulose membrane. 50ng of Hydroxy-peptide or Non Hydroxy-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Hydroxy-HIF1A-P564 Antibody (ABIN650844) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Linke, Stojkoski, Kewley et al.: "Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14391-7, 2004 (PubMed).