SRY (Sex Determining Region Y)-Box 2 (SOX2) (pSer250) antibody

Antigen: SRY (Sex Determining Region Y)-Box 2 (SOX2)

Synonyms: ANOP3, MCOPS3, MGC2413, RGD1565646, SOX2, MGC148683, lcc, ysb, Sox-2

Binding Site: pSer250

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650874

Additional Information

Alternative name: Sox2

Gene ID: 6657

UniProt: NP_003097.1, P48431

Immunogen: This phospho-Sox2-pS250 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 230-260 surrounding S250 of human Sox2.

Isotype: Ig

Description: Other names: SOX2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This phospho-Sox2-pS250 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 230-260 surrounding S250 of human Sox2.

Molecular Weight: 34310 DA

Comments:

Background: This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blot 1:500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Dot blot analysis of anti-phospho-Sox2-pS250 Phospho-specific Pab (ABIN650874) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Phospho-Sox2-pS250 Antibody (ABIN650874) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Phospho-Sox2-pS250 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Phospho-Sox2-pS250 Antibody (ABIN650874) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Kamachi, Uchikawa, Kondoh: "Pairing SOX off: with partners in the regulation of embryonic development." in: Trends in genetics : TIG, Vol. 16, Issue 4, pp. 182-7, 2000 (PubMed).

Laga, Lai, Zhan et al.: "Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology." in: The American journal of pathology, Vol. 176, Issue 2, pp. 903-13, 2010 (PubMed).