APG8b (MAP1LC3B) (Cleaved) antibody

Antigen: APG8b (MAP1LC3B)

Binding Site: Cleaved

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650885

Additional Information

Gene ID: 81631

UniProt: NP_073729, Q9GZQ8

Immunogen: This Cleaved-APG8b (MAP1LC3B) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human APG8b.

Isotype: Ig

Description: Other names: Microtubule-associated proteins 1A/1B light chain 3B,MAP1LC3B,MAP1ALC3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Cleaved-APG8b (MAP1LC3B) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human APG8b.

Molecular Weight: 14688 DA

Comments:

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Dot blot~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Autophagy

Restrictions: For Research Use only

Images

Dot blot analysis of anti-Cleaved-APG8b (MAP1LC3B) Phospho-specific Pab (ABIN650885) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Publications

Product: Kirkin, Lamark, Sou et al.: "A role for NBR1 in autophagosomal degradation of ubiquitinated substrates." in: Molecular cell, Vol. 33, Issue 4, pp. 505-16, 2009 (PubMed).