F113A (C-Term) antibody

Antigen: F113A

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650927

Additional Information

Gene ID: 64773

UniProt: Q9H1Q7

Immunogen: This F113A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285~314 amino acids from the C-terminal region of human F113A.

Isotype: Ig

Description: Other names: Sarcoma antigen NY-SAR-23, Protein FAM113A,C20orf81, FAM113A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This F113A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285~314 amino acids from the C-terminal region of human F113A.

Molecular Weight: 51782 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of F113A Antibody (C-term) (ABIN650927) in K562 cell line lysates (35ug/lane). F113A (arrow) was detected using the purified Pab.

F113A Antibody (C-term) (ABIN650927) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the F113A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

F113A Antibody (C-term) (ABIN650927) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Lee, Obata, Yoshida et al.: "Immunomic analysis of human sarcoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2651-6, 2003 (PubMed).