BT2A2 (C-Term) antibody

Antigen: BT2A2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650936

Additional Information

Gene ID: 10385

UniProt: Q8WVV5

Immunogen: This BT2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335~365 amino acids from the C-terminal region of human BT2A2.

Isotype: Ig

Description: Other names: Butyrophilin subfamily 2 member A2,BTF2, BT2.2, BTN2A2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BT2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335~365 amino acids from the C-terminal region of human BT2A2.

Molecular Weight: 59070 DA

Comments:

Background: BT2A2 is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type 1 receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of BT2A2 Antibody (C-term) (ABIN650936) in MDA-MB435 cell line lysates (35ug/lane). BT2A2 (arrow) was detected using the purified Pab.

BT2A2 Antibody (C-term) (ABIN650936) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Mungall, Palmer, Sims et al.: "The DNA sequence and analysis of human chromosome 6." in: Nature, Vol. 425, Issue 6960, pp. 805-11, 2003 (PubMed).

Shi, Levinson, Duan et al.: "Common variants on chromosome 6p22.1 are associated with schizophrenia." in: Nature, Vol. 460, Issue 7256, pp. 753-7, 2009 (PubMed).