O13H1 (C-Term) antibody

Antigen: O13H1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650965

Additional Information

Gene ID: 347468

UniProt: Q8NG92

Immunogen: This O13H1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the C-terminal region of human O13H1.

Isotype: Ig

Description: Other names: Olfactory receptor ORX-1, Olfactory receptor 13H1,OR13H1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This O13H1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279~308 amino acids from the C-terminal region of human O13H1.

Molecular Weight: 34570 DA

Comments:

Background: O13H1 interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of O13H1 Antibody (C-term) (ABIN650965) in Jurkat cell line lysates (35ug/lane). O13H1 (arrow) was detected using the purified Pab.

O13H1 Antibody (C-term) (ABIN650965) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Gilad, Bustamante, Lancet et al.: "Natural selection on the olfactory receptor gene family in humans and chimpanzees." in: American journal of human genetics, Vol. 73, Issue 3, pp. 489-501, 2003 (PubMed).

Malnic, Godfrey, Buck: "The human olfactory receptor gene family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2584-9, 2004 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).