DBF4 Homolog B (S. Cerevisiae) (DBF4B) (Center) antibody

Antigen: DBF4 Homolog B (S. Cerevisiae) (DBF4B)

Synonyms: DRF1, ASKL1, chifb, ZDBF1B, FLJ13087, MGC15009, dbf4, Drf1, dbf4b, DBF4B

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651018

Additional Information

Alternative name: DBF4B

Gene ID: 80174

UniProt: Q8NFT6

Immunogen: This DBF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the Central region of human DBF4B.

Isotype: Ig

Description: Other names: Chiffon homolog B, Dbf4-related factor 1, Activator of S phase kinase-like protein 1, ASK-like protein 1, Protein DBF4 homolog B,DRF1, ASKL1, DBF4B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DBF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~254 amino acids from the Central region of human DBF4B.

Molecular Weight: 67243 DA

Comments:

Background: DBF4B encodes a regulator of the CDC7-like 1 protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and its expression is cell cycle-regulated. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DBF4B Antibody (Center) (ABIN651018) in MDA-MB435 cell line lysates (35ug/lane). DBF4B (arrow) was detected using the purified Pab.

DBF4B Antibody (Center) (ABIN651018) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Montagnoli, Bosotti, Villa et al.: "Drf1, a novel regulatory subunit for human Cdc7 kinase." in: The EMBO journal, Vol. 21, Issue 12, pp. 3171-81, 2002 (PubMed).

Yoshizawa-Sugata, Ishii, Taniyama et al.: "A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phases." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 13062-70, 2005 (PubMed).

Tsuji, Lau, Chiang et al.: "The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control." in: Molecular cell, Vol. 32, Issue 6, pp. 862-9, 2008 (PubMed).