Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC) (C-Term) antibody

Antigen: Eukaryotic Elongation Factor, Selenocysteine-tRNA-Specific (EEFSEC)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651045

Additional Information

Alternative name: EEFSEC

Gene ID: 60678

UniProt: P57772

Immunogen: This EEFSEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553~582 amino acids from the C-terminal region of human EEFSEC.

Isotype: Ig

Description: Other names: Eukaryotic elongation factor, selenocysteine-tRNA-specific, Elongation factor sec, Selenocysteine-specific elongation factor,SELB, EEFSEC

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This EEFSEC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553~582 amino acids from the C-terminal region of human EEFSEC.

Molecular Weight: 65305 DA

Comments:

Background: EEFSEC is translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EEFSEC Antibody (C-term) (ABIN651045) in Hela cell line lysates (35ug/lane). EEFSEC (arrow) was detected using the purified Pab.

EEFSEC Antibody (C-term) (ABIN651045) IHC analysis in formalin fixed and paraffin embedded tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EEFSEC Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

EEFSEC Antibody (C-term) (ABIN651045) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Muzny, Scherer, Kaul et al.: "The DNA sequence, annotation and analysis of human chromosome 3." in: Nature, Vol. 440, Issue 7088, pp. 1194-8, 2006 (PubMed).

Donovan, Caban, Ranaweera et al.: "A novel protein domain induces high affinity selenocysteine insertion sequence binding and elongation factor recruitment." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 35129-39, 2008 (PubMed).

Gudmundsson, Sulem, Gudbjartsson et al.: "Genome-wide association and replication studies identify four variants associated with prostate cancer susceptibility." in: Nature genetics, Vol. 41, Issue 10, pp. 1122-6, 2009 (PubMed).