ATH1, Acid Trehalase-Like 1 (Yeast) (ATHL1) (C-Term) antibody

Antigen: ATH1, Acid Trehalase-Like 1 (Yeast) (ATHL1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651175

Additional Information

Alternative name: ATHL1

Gene ID: 80162

UniProt: NP_079368

Immunogen: This ATHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 695-725 from the C-terminal region of human ATHL1.

Isotype: Ig

Description: Other names: Acid trehalase-like protein 1，,ATHL1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 695-725 from the C-terminal region of human ATHL1.

Molecular Weight: 80655 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ATHL1 Antibody (C-term) (ABIN651175) in MDA-MB435 cell line lysates (35ug/lane). ATHL1 (arrow) was detected using the purified Pab.

ATHL1 Antibody (C-term) (ABIN651175) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATHL1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ATHL1 Antibody (C-term) (ABIN651175) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).