Origin Recognition Complex, Subunit 1-Like (Yeast) (ORC1L) (Center) antibody

Antigen: Origin Recognition Complex, Subunit 1-Like (Yeast) (ORC1L)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651187

Additional Information

Alternative name: ORC1L

Gene ID: 4998

UniProt: Q13415

Immunogen: This ORC1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-315 amino acids from the Central region of human ORC1L.

Isotype: Ig

Description: Other names: Replication control protein 1, Origin recognition complex subunit 1,PARC1, ORC1, ORC1L

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ORC1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-315 amino acids from the Central region of human ORC1L.

Molecular Weight: 97350 DA

Comments:

Background: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Stem Cells, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ORC1L Antibody (Center) (ABIN651187) in MCF-7 cell line lysates (35ug/lane).ORC1L (arrow) was detected using the purified Pab.

Publications

Product: Hemerly, Prasanth, Siddiqui et al.: "Orc1 controls centriole and centrosome copy number in human cells." in: Science (New York, N.Y.), Vol. 323, Issue 5915, pp. 789-93, 2009 (PubMed).