DDHD Domain Containing 2 (DDHD2) (N-Term) antibody

Antigen: DDHD Domain Containing 2 (DDHD2)

Synonyms: SAMWD1, KIAA0725, mKIAA0725, 2010305K11Rik, MGC139315

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651215

Additional Information

Alternative name: DDHD2

Gene ID: 23259

UniProt: O94830

Immunogen: This DDHD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-140 amino acids from the N-terminal region of human DDHD2.

Isotype: Ig

Description: Other names: SAM, WWE and DDHD domain-containing protein 1, DDHD domain-containing protein 2, Phospholipase DDHD2,SAMWD1, KIAA0725, DDHD2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDHD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-140 amino acids from the N-terminal region of human DDHD2.

Molecular Weight: 81032 DA

Comments:

Background: DDHD2 is phospholipase that hydrolyzes preferentially phosphatidic acid and phosphatidylethanolamine. DDHD2 may be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DDHD2 Antibody (N-term) (ABIN651215) in K562 cell line lysates (35ug/lane).DDHD2 (arrow) was detected using the purified Pab.

DDHD2 Antibody (N-term) (ABIN651215) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDHD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DDHD2 Antibody (N-term) (ABIN651215) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Nakajima, Sonoda, Mizoguchi et al.: "A novel phospholipase A1 with sequence homology to a mammalian Sec23p-interacting protein, p125." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 11329-35, 2002 (PubMed).

Kwek, Roy, Zhou et al.: "Co-amplified genes at 8p12 and 11q13 in breast tumors cooperate with two major pathways in oncogenesis." in: Oncogene, Vol. 28, Issue 17, pp. 1892-903, 2009 (PubMed).