Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (C-Term), (AA 541-568) antibody

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Antigen
Synonyms GRP78, BIP, MIF2, grp78, hspa5a, BiP, GRP-78, HSPA5
Epitope
C-Term, AA 541-568
(10), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(97), (59), (54), (7), (7), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(81), (26), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(97), (48), (28), (25), (18), (13), (10), (5), (3), (1), (1)
Pubmed 2 references available
Catalog no. ABIN651231
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-568 AA from the C-terminal region of human HSPA5.
Clone RB20814
Isotype Ig
Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-587 amino acids from the C-terminal region of human HSPA5.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Pig (Porcine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HSPA5
Background When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Synonyms: HSPA5,GRP78,78 kDa glucose-regulated protein
Molecular Weight 72333 DA
Gene ID 3309
UniProt NP_005338, P11021
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (C-Term), (AA 541-568) antibody HSPA5 Antibody (C-term) (ABIN651231) western blot analysis in A375,A2058,Hela cell line lysates (35 µg/lane). This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (C-Term), (AA 541-568) antibody (2) HSPA5 Antibody (C-term) (ABIN651231) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (C-Term), (AA 541-568) antibody (3) HSPA5 Antibody (C-term) (ABIN651231) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Zhuang, Scolyer, Murali et al.: "Lactate dehydrogenase 5 expression in melanoma increases with disease progression and is associated with expression of Bcl-XL and Mcl-1, but not Bcl-2 proteins." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 23, Issue 1, pp. 45-53, 2010 (PubMed).

Zhao, Zhang, Tian et al.: "Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis." in: Journal of medical virology, Vol. 82, Issue 1, pp. 14-22, 2009 (PubMed).

Hosts (81), (26), (4)
Reactivities (97), (59), (54), (7), (7), (6), (6), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Applications (97), (48), (28), (25), (18), (13), (10), (5), (3), (1), (1)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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