Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 261-289), (Center) antibody

Details for Product No. ABIN651232
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
GRP78, BiP, GRP-78, grp78, hspa5a, BIP, MIF2, AL022860, AU019543, Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, SEZ-7, Sez7, baffled, mBiP, cb865, fb60h09, fi36d04, wu:fb60h09, wu:fi36d04, zgc:55994, zgc:7 ... show more
GRP78, BiP, GRP-78, grp78, hspa5a, BIP, MIF2, AL022860, AU019543, Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, SEZ-7, Sez7, baffled, mBiP, cb865, fb60h09, fi36d04, wu:fb60h09, wu:fi36d04, zgc:55994, zgc:77606 show less
Epitope
AA 261-289, Center
(26), (15), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Zebrafish (Danio rerio)
(170), (72), (60), (7), (6), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host
Rabbit
(139), (61), (4), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(9), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(191), (94), (62), (34), (24), (20), (10), (10), (9), (8), (6), (3), (1), (1), (1)
Pubmed 2 references available
Quantity 400 μL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN651232
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 AA from the Central region of human HSPA5.
Clone RB20813
Isotype Ig
Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSPA5.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HSPA5
Background When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Synonyms: Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Immunoglobulin heavy chain-binding protein, BiP, Heat shock 70 kDa protein 5, 78 kDa glucose-regulated protein, GRP-78,GRP78, HSPA5
Molecular Weight 72333 DA
Gene ID 3309
UniProt P11021
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Stem Cells, Protein Modifications, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 261-289), (Center) antibody HSPA5 Antibody (Center) (ABIN651232) western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35 µg/lane). This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 261-289), (Center) antibody (2) HSPA5 Antibody (Center) (ABIN651232) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining
Background publications Zhuang, Scolyer, Murali et al.: "Lactate dehydrogenase 5 expression in melanoma increases with disease progression and is associated with expression of Bcl-XL and Mcl-1, but not Bcl-2 proteins." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 23, Issue 1, pp. 45-53, 2010 (PubMed).

Zhao, Zhang, Tian et al.: "Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis." in: Journal of medical virology, Vol. 82, Issue 1, pp. 14-22, 2009 (PubMed).

Validation Images
Did you look for something else?
back to top