GRP78 antibody (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa)) (AA 261-289)

Details for Product anti-HSPA5 Antibody No. ABIN651232, Supplier: Log in to see
Antigen
  • GRP78
  • BiP
  • GRP-78
  • grp78
  • hspa5a
  • BIP
  • MIF2
  • AL022860
  • AU019543
  • Bip
  • D2Wsu141e
  • D2Wsu17e
  • Grp78
  • Hsce70
  • SEZ-7
  • Sez7
  • baffled
  • mBiP
  • cb865
  • fb60h09
  • fi36d04
  • wu:fb60h09
  • wu:fi36d04
  • zgc:55994
  • zgc:77606
  • 78 kDa glucose-regulated protein
  • heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
  • BiP/GRP78
  • glucose-regulated protein 78
  • Hsp70 family ATPase KAR2
  • glucose-regulated protein 78kDa
  • heat shock 70 kDa protein 5a
  • heat shock protein 5
  • CpipJ_CPIJ003550
  • HSPA5
  • grp78
  • LOC100533358
  • BiP/grp78
  • Tc00.1047053506585.40
  • Tb11.02.5450
  • Tb11.02.5500
  • LmjF28.1200
  • KAR2
  • hspa5
  • Hspa5
Alternatives
anti-Zebrafish (Danio rerio) GRP78 antibody for Immunofluorescence
Epitope
AA 261-289, Center
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41
29
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21
11
11
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9
7
5
5
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3
3
3
2
2
2
1
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1
1
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1
Reactivity
Human, Zebrafish (Danio rerio)
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329
304
65
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58
54
31
15
14
6
4
3
3
3
2
2
1
1
1
1
Host
Rabbit
293
201
7
6
3
1
Clonality (Clone)
Polyclonal ()
Conjugate
This GRP78 antibody is un-conjugated
31
26
20
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13
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11
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11
11
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3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
474
232
221
217
149
59
35
26
13
13
11
7
6
6
3
3
2
Options
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Immunogen This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 AA from the Central region of human HSPA5.
Clone RB20813
Isotype Ig
Specificity This HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSPA5.
Predicted Reactivity Cow (Bovine),Chicken,Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HSPA5 (HSPA5 Antibody Abstract)
Background When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
Synonyms: Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Immunoglobulin heavy chain-binding protein, BiP, Heat shock 70 kDa protein 5, 78 kDa glucose-regulated protein, GRP-78,GRP78, HSPA5
Molecular Weight 72333 DA
Gene ID 3309
UniProt P11021
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Stem Cells, Protein Modifications, Chromatin, Cell Structure
Pathways Thyroid Hormone Synthesis, ER-Nucleus Signaling
Application Notes WB = 1:1000, IHC (p) = 1:50-100, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-GRP78 antibody (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa)) (AA 261-289) (ABIN651232) HSPA5 Antibody (Center) (ABIN651232) western blot analysis in Hela,A2058,NCI-H460 cel...
Immunohistochemistry (IHC) image for anti-GRP78 antibody (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa)) (AA 261-289) (ABIN651232) HSPA5 Antibody (Center) (ABIN651232) IHC analysis in formalin fixed and paraffin embe...
Background publications Zhuang, Scolyer, Murali, McCarthy, Zhang, Thompson, Hersey: "Lactate dehydrogenase 5 expression in melanoma increases with disease progression and is associated with expression of Bcl-XL and Mcl-1, but not Bcl-2 proteins." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 23, Issue 1, pp. 45-53, 2010 (PubMed).

Zhao, Zhang, Tian, Fang, Lu, Yuan, Yang, Wen: "Proteomic analysis of cell lines expressing small hepatitis B surface antigen revealed decreased glucose-regulated protein 78 kDa expression in association with higher susceptibility to apoptosis." in: Journal of medical virology, Vol. 82, Issue 1, pp. 14-22, 2009 (PubMed).