Helicase, Lymphoid-Specific (HELLS) (N-Term) antibody

Antigen: Helicase, Lymphoid-Specific (HELLS)

Synonyms: LSH, PASG, SMARCA6, FLJ10339, Nbla10143, HELLS, cb65, pasg, sb:cb65, sb:cb749, im:6911667

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651287

Additional Information

Alternative name: HELLS

Gene ID: 3070

UniProt: Q9NRZ9, NP_060533

Immunogen: This HELLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human HELLS.

Isotype: Ig

Description: Other names: Lymphoid-specific helicase,HELLS,SMARCA6, PASG

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HELLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human HELLS.

Molecular Weight: 97074 DA

Comments:

Background: HELLS is a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

HELLS Antibody (N-term) (ABIN651287) western blot analysis in CEM cell line lysates (15ug/lane). This demonstrates the HELLS antibody detected the HELLS protein (arrow).

HELLS Antibody (N-term) (ABIN651287) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Suzuki, Farrar, Yegnasubramanian et al.: "Stable knockdown of PASG enhances DNA demethylation but does not accelerate cellular senescence in TIG-7 human fibroblasts." in: Epigenetics : official journal of the DNA Methylation Society, Vol. 3, Issue 5, pp. 281-91, 2008 (PubMed).

Palmieri, Wilson, Iversen et al.: "Polymorphism in the IL18 gene and epithelial ovarian cancer in non-Hispanic white women." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 17, Issue 12, pp. 3567-72, 2008 (PubMed).

Zhou, Han, Li et al.: "Senescence delay and repression of p16INK4a by Lsh via recruitment of histone deacetylases in human diploid fibroblasts." in: Nucleic acids research, Vol. 37, Issue 15, pp. 5183-96, 2009 (PubMed).