Chromosome 13 Open Reading Frame 27 (C13orf27) (Center) antibody

Antigen: Chromosome 13 Open Reading Frame 27 (C13orf27)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651306

Additional Information

Alternative name: C13orf27

Gene ID: 93081

UniProt: Q5JUR7

Immunogen: This C13orf27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-155 amino acids from the Central region of human C13orf27.

Isotype: Ig

Description: Other names: Uncharacterized protein C13orf27,C13orf27

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C13orf27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-155 amino acids from the Central region of human C13orf27.

Molecular Weight: 25585 DA

Comments:

Background: The exact function of this protein is unknow.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of C13orf27 Antibody (Center) (ABIN651306) in Jurkat cell line lysates (35ug/lane).C13orf27 (arrow) was detected using the purified Pab.

C13orf27 Antibody (Center) (ABIN651306) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

C13orf27 Antibody (Center) (ABIN651306) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Dunham, Matthews, Burton et al.: "The DNA sequence and analysis of human chromosome 13." in: Nature, Vol. 428, Issue 6982, pp. 522-8, 2004 (PubMed).