Intraflagellar Transport 20 Homolog (Chlamydomonas) (IFT20) (Center) antibody

Antigen: Intraflagellar Transport 20 Homolog (Chlamydomonas) (IFT20)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651326

Additional Information

Alternative name: IFT20

Gene ID: 90410

UniProt: Q8IY31

Immunogen: This IFT20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the Central region of human IFT20.

Isotype: Ig

Description: Other names: Intraflagellar transport protein 20 homolog, hIFT20,IFT20

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IFT20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the Central region of human IFT20.

Molecular Weight: 15281 DA

Comments:

Background: IFT20 is part of intraflagellar transport (IFT) particles involved in ciliary process assembly. IFT20 may play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of IFT20 Antibody (Center) (ABIN651326) in mouse lung tissue lysates (35ug/lane).IFT20 (arrow) was detected using the purified Pab.

IFT20 Antibody (Center) (ABIN651326) IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IFT20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Jurczyk, Gromley, Redick et al.: "Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly." in: The Journal of cell biology, Vol. 166, Issue 5, pp. 637-43, 2004 (PubMed).

Follit, Tuft, Fogarty et al.: "The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly." in: Molecular biology of the cell, Vol. 17, Issue 9, pp. 3781-92, 2006 (PubMed).