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Histone H1.5 (AA 33-60), (N-Term) antibody

Details for Product No. ABIN651424, Supplier: Log in to see
Antigen
  • H1
  • H1.5
  • H1B
  • H1F5
  • H1s-3
  • H1f5
Epitope
AA 33-60, N-Term
17
15
1
1
Reactivity
Human
23
2
1
1
Host
Rabbit
23
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
21
16
14
2
2
1
1
1
Supplier
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Immunogen This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-60 AA from the N-terminal region of human HIST1H1B.
Clone RB30034
Isotype Ig
Specificity This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human HIST1H1B.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name HIST1H1B
Background Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.
Synonyms: Histone H1a, Histone H1.5,H1F5, HIST1H1B
Molecular Weight 22580 DA
Gene ID 3009
UniProt P16401
Research Area Cancer
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Histone H1.5 (AA 33-60), (N-Term) antibody (ABIN651424) Western blot analysis of HIST1H1B Antibody (N-term) (ABIN651424) in NCI-H460 cell lin...
Flow Cytometry (FACS) image for anti-Histone H1.5 (AA 33-60), (N-Term) antibody (ABIN651424) HIST1H1B Antibody (N-term) (ABIN651424) flow cytometric analysis of NCI-H460 cells (r...
Background publications Happel, Stoldt, Schmidt et al.: "M phase-specific phosphorylation of histone H1.5 at threonine 10 by GSK-3." in: Journal of molecular biology, Vol. 386, Issue 2, pp. 339-50, 2009 (PubMed).

Trojer, Li, Sims et al.: "L3MBTL1, a histone-methylation-dependent chromatin lock." in: Cell, Vol. 129, Issue 5, pp. 915-28, 2007 (PubMed).