UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 5 (B4GALT5) (C-Term), (AA 324-350) antibody

Details for Product No. ABIN651558
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Antigen
Synonyms B4Gal-T5, BETA4-GALT-IV, beta4Gal-T5, beta4GalT-V, gt-V, 9430078I07Rik, AW049941, AW539721
Epitope
C-Term, AA 324-350
(15), (7), (5), (1), (1)
Reactivity
Human, Mouse (Murine)
(28), (13)
Host
Rabbit
(22), (7)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS)
(29), (21), (8), (1)
Pubmed 8 references available
Quantity 400 μL
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Catalog No. ABIN651558
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Immunogen This B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 AA from the C-terminal region of human B4GALT5.
Clone RB25986
Isotype Ig
Specificity This B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human B4GALT5.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name B4GALT5
Background This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq].
Synonyms: B4GALT5,
Molecular Weight 45119 DA
Gene ID 9334
NCBI Accession NP_004767
UniProt O43286
Research Area Cell Signaling, Cell Structure, Protein Modifications
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 5 (B4GALT5) (C-Term), (AA 324-350) antibody B4GALT5 Antibody (C-term)(ABIN651558) western blot analysis in mouse heart tissue lysates (35 µg/lane). This demonstrates the B4GALT5 antibody detected B4GALT5 protein (arrow).
anti-UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 5 (B4GALT5) (C-Term), (AA 324-350) antibody (2) B4GALT5 Antibody (C-term) (ABIN651558) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Sato, Furukawa, Bakker et al.: "Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 472-7, 1998 (PubMed).

Amado, Almeida, Schwientek et al.: "Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." in: Biochimica et biophysica acta, Vol. 1473, Issue 1, pp. 35-53, 2000 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Xu, Zhang, Chen et al.: "Over-expression of beta-1,4-galactosyltransferase V increases the growth of astrocytoma cell line." in: Journal of experimental & clinical cancer research : CR, Vol. 21, Issue 3, pp. 409-14, 2002 (PubMed).

Sato, Furukawa: "Transcriptional regulation of the human beta-1,4-galactosyltransferase V gene in cancer cells: essential role of transcription factor Sp1." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39574-83, 2004 (PubMed).

Jiang, Chen, Shen et al.: "Beta1,4-galactosyltransferase V functions as a positive growth regulator in glioma." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9482-9, 2006 (PubMed).

Sato, Furukawa: "Sequential action of Ets-1 and Sp1 in the activation of the human beta-1,4-galactosyltransferase V gene involved in abnormal glycosylation characteristic of cancer cells." in: The Journal of biological chemistry, Vol. 282, Issue 38, pp. 27702-12, 2007 (PubMed).

Kitayama, Hayashida, Nishida et al.: "Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30085-96, 2007 (PubMed).

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