TM2 Domain Containing 1 (TM2D1) (N-Term) antibody

Antigen: TM2 Domain Containing 1 (TM2D1)

Synonyms: si:ch211-112f11.3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651573

Additional Information

Alternative name: TM2D1

Gene ID: 83941

UniProt: Q9BX74, NP_114416.1

Immunogen: This TM2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human TM2D1.

Isotype: Ig

Description: Other names: TM2D1,BBP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TM2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human TM2D1.

Molecular Weight: 22327 DA

Comments:

Background: The protein encoded by this gene is a beta-amyloid peptide-binding protein. It contains a structural module related to that of the seven transmembrane domain G protein-coupled receptor superfamily and known to be important in heterotrimeric G protein activation. Beta-amyloid peptide has been established to be a causative factor in neuron death and the consequent diminution of cognitive abilities observed in Alzheimer's disease. This protein may be a target of neurotoxic beta-amyloid peptide, and may mediate cellular vulnerability to beta-amyloid peptide toxicity through a G protein-regulated program of cell death.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

TM2D1 Antibody (N-term)(ABIN651573) western blot analysis in HL-60 cell line lysates (35ug/lane). This demonstrates the TM2D1 antibody detected the TM2D1 protein (arrow).

TM2D1 Antibody (N-term) (ABIN651573) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Kajkowski, Lo, Ning et al.: "beta -Amyloid peptide-induced apoptosis regulated by a novel protein containing a g protein activation module." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18748-56, 2001 (PubMed).

Cam, Zerbinatti, Knisely et al.: "The low density lipoprotein receptor-related protein 1B retains beta-amyloid precursor protein at the cell surface and reduces amyloid-beta peptide production." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 29639-46, 2004 (PubMed).