Cullin 3 (CUL3) (C-Term) antibody

Antigen: Cullin 3 (CUL3)

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651617

Additional Information

Alternative name: CUL3

Gene ID: 8452

UniProt: Q13618, NP_003581.1

Immunogen: This CUL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 743-772 amino acids from the C-terminal region of human CUL3.

Isotype: Ig

Description: Other names: CUL3,KIAA0617

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CUL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 743-772 amino acids from the C-terminal region of human CUL3.

Molecular Weight: 88930 DA

Comments:

Background: CUL3 is a component of a ubiquitin E3 ligase that is essential for mitotic division (Sumara et al., 2007 [PubMed 17543862]).

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

CUL3 Antibody (C-term)(ABIN651617) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the CUL3 antibody detected the CUL3 protein (arrow).

CUL3 Antibody (C-term) (ABIN651617) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Du, Sansores-Garcia, Zu et al.: "Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family." in: The Journal of biological chemistry, Vol. 273, Issue 38, pp. 24289-92, 1998 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Singer, Gurian-West, Clurman et al.: "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells." in: Genes & development, Vol. 13, Issue 18, pp. 2375-87, 1999 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Chen, Yang, Meng et al.: "Cullin mediates degradation of RhoA through evolutionarily conserved BTB adaptors to control actin cytoskeleton structure and cell movement." in: Molecular cell, Vol. 35, Issue 6, pp. 841-55, 2009 (PubMed).

Zhuang, Calabrese, Liu et al.: "Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases." in: Molecular cell, Vol. 36, Issue 1, pp. 39-50, 2009 (PubMed).

Maerki, Olma, Staubli et al.: "The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis." in: The Journal of cell biology, Vol. 187, Issue 6, pp. 791-800, 2009 (PubMed).