Ectonucleoside Triphosphate diphosphohydrolase 7 (ENTPD7) (Center) antibody

Antigen: Ectonucleoside Triphosphate diphosphohydrolase 7 (ENTPD7)

Synonyms: LALP1, NTPDase7

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651728

Additional Information

Alternative name: ENTPD7

Gene ID: 57089

UniProt: NP_065087.1, Q9NQZ7

Immunogen: This ENTPD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 356-386 amino acids from the Central region of human ENTPD7.

Isotype: Ig

Description: Other names: Ectonucleoside triphosphate diphosphohydrolase 7,ENTPD7,LALP1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ENTPD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 356-386 amino acids from the Central region of human ENTPD7.

Molecular Weight: 68960 DA

Comments:

Background: The ENTPD7 belongs to the GDA1/CD39 NTPase family and the gene resides on chromosome 10q23-q24 and contains 12 exons and 11 introns covering a genomic region of approximately 46 kilobase pairs. The subcellular localization and enzymatic activity of ENTPD7 indicated that ENTPD7 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, GTP, and CTP.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Restrictions: For Research Use only

Images

ENTPD7 Antibody (Center) (ABIN651728) western blot analysis in Hela,HepG2 cell line lysates (15ug/lane). This demonstrates the ENTPD7 antibody detected the ENTPD7 protein (arrow).

ENTPD7 Antibody (Center) (ABIN651728) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENTPD7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ENTPD7 Antibody (Center) (ABIN651728) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Shi, Kukar, Wang et al.: "Molecular cloning and characterization of a novel mammalian endo-apyrase (LALP1)." in: The Journal of biological chemistry, Vol. 276, Issue 20, pp. 17474-8, 2001 (PubMed).

Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).