BUD31 Homolog (S. Cerevisiae) (BUD31) (Center) antibody

Antigen: BUD31 Homolog (S. Cerevisiae) (BUD31)

Synonyms: g10l, zgc:110615, BUD31, MGC137430, CWC14, LOC100221237, bud31, G10, EDG2, EDG-2, fSAP17, YCR063W, MGC111202, Edg2, C77604, g10, MGC52613, c77604

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651732

Additional Information

Alternative name: BUD31

Gene ID: 8896

UniProt: P41223, NP_003901.2

Immunogen: This BUD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the Central region of human BUD31.

Isotype: Ig

Description: Other names: Protein BUD31 homolog,BUD31,EDG2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BUD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-77 amino acids from the Central region of human BUD31.

Molecular Weight: 17000 DA

Comments:

Background: BUD31, also known as EDG2, is the homolog of BUD31 from Saccharomyces cerevisiae.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

BUD31 Antibody (Center) (ABIN651732) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the BUD31 antibody detected the BUD31 protein (arrow).

BUD31 Antibody (Center) (ABIN651732) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Hla, Jackson, Appleby et al.: "Characterization of edg-2, a human homologue of the Xenopus maternal transcript G10 from endothelial cells." in: Biochimica et biophysica acta, Vol. 1260, Issue 2, pp. 227-9, 1995 (PubMed).

Scherer, Cheung, MacDonald et al.: "Human chromosome 7: DNA sequence and biology." in: Science (New York, N.Y.), Vol. 300, Issue 5620, pp. 767-72, 2003 (PubMed).

Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).