Cell Division Cycle 26 Homolog (S. Cerevisiae) (CDC26) (C-Term) antibody

Antigen: Cell Division Cycle 26 Homolog (S. Cerevisiae) (CDC26)

Synonyms: MGC130281, 2010012C09Rik, apc12, zgc:100965, MGC127671, HIT3, SCD26, DKFZp468D207

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651734

Additional Information

Alternative name: CDC26

Gene ID: 246184

UniProt: NP_644815.1, Q8NHZ8

Immunogen: This CDC26 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the C-terminal region of human CDC26.

Isotype: Ig

Description: Other names: Anaphase-promoting complex subunit CDC26,CDC26,C9orf17

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC26 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the C-terminal region of human CDC26.

Molecular Weight: 9777 DA

Comments:

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

CDC26 Antibody (C-term) (ABIN651734) western blot analysis in HL-60 cell line lysates (15ug/lane). This demonstrates the CDC26 antibody detected the CDC26 protein (arrow).

CDC26 Antibody (C-term) (ABIN651734) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

Dube, Herzog, Gieffers et al.: "Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C." in: Molecular cell, Vol. 20, Issue 6, pp. 867-79, 2005 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).