Glucosidase, Alpha; Neutral AB (GANAB) (N-Term) antibody

Antigen: Glucosidase, Alpha; Neutral AB (GANAB)

Synonyms: G2AN, GLUII, KIAA0088, G2an, GANAB, Hbg1, GB12607

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651840

Additional Information

Alternative name: GANAB

Gene ID: 23193

UniProt: NP_938148.1, Q14697

Immunogen: This GANAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human GANAB.

Isotype: Ig

Description: Other names: Neutral alpha-glucosidase AB,GANAB,KIAA0088, G2AN

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GANAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human GANAB.

Molecular Weight: 106874 DA

Comments:

Background: Glucosidase II is an endoplasmic reticulum localized enzyme that has an important function in the folding and maturation of glycoproteins. Glucosidase II is composed of two soluble proteins: a catalytic alpha-subunit and a beta-subunit of unknown function, both of which are highly conserved in mammals.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

GANAB Antibody (N-term) (ABIN651840) western blot analysis in MDA-MB435 cell line lysates (15ug/lane). This demonstrates the GANAB antibody detected the GANAB protein (arrow).

GANAB Antibody (N-term) (ABIN651840) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane). This demonstrates the GANAB antibody detected the GANAB protein (arrow).

Publications

Product: Martiniuk, Ellenbogen, Hirschhorn: "Identity of neutral alpha-glucosidase AB and the glycoprotein processing enzyme glucosidase II. Biochemical and genetic studies." in: The Journal of biological chemistry, Vol. 260, Issue 2, pp. 1238-42, 1985 (PubMed).

Martiniuk, Smith, Ellenbogen et al.: "Assignment of the gene for neutral alpha-glucosidase AB to chromosome 11." in: Cytogenetics and cell genetics, Vol. 35, Issue 2, pp. 110-6, 1983 (PubMed).

Trombetta, Simons, Helenius: "Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit." in: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27509-16, 1996 (PubMed).