Z280D (C-Term) antibody

Antigen: Z280D

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651906

Additional Information

Gene ID: 54816

UniProt: NP_060131.2, Q6N043

Immunogen: This Z280D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 958-987 amino acids from the C-terminal region of human Z280D.

Isotype: Ig

Description: Other names: Zinc finger protein 280D,ZNF280D,SUHW4, KIAA1584

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Z280D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 958-987 amino acids from the C-terminal region of human Z280D.

Molecular Weight: 109285 DA

Comments:

Background: ZNF280D contains five C2H2-type zinc fingers and may function as a transcription factor. There are five named isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Z280D Antibody (C-term) (ABIN651906) western blot analysis in A549,NCI-H460 cell line lysates (35ug/lane). This demonstrates the Z280D antibody detected the Z280D protein (arrow).

Z280D Antibody (C-term) (ABIN651906) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).