RM44 (N-Term) antibody

Antigen: RM44

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651913

Additional Information

Gene ID: 65080

UniProt: Q9H9J2, NP_075066.1

Immunogen: This RM44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human RM44.

Isotype: Ig

Description: Other names: 39S ribosomal protein L44, mitochondrial,MRPL44,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RM44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human RM44.

Molecular Weight: 37535 DA

Comments:

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

RM44 Antibody (N-term) (ABIN651913) western blot analysis in 293 cell line lysates (35ug/lane). This demonstrates the RM44 antibody detected the RM44 protein (arrow).

RM44 Antibody (N-term) (ABIN651913) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RM44 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

RM44 Antibody (N-term) (ABIN651913) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Koc, Burkhart, Blackburn et al.: "The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 43958-69, 2001 (PubMed).

Zhang, Gerstein: "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome." in: Genomics, Vol. 81, Issue 5, pp. 468-80, 2003 (PubMed).