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RM44 (N-Term) antibody
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|2 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This RM44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human RM44.|
|Description||Other names: 39S ribosomal protein L44, mitochondrial,MRPL44,|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This RM44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human RM44.|
|Molecular Weight||37535 DA|
Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq].
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Chromatin, Signaling, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
Koc, Burkhart, Blackburn et al.: "The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 43958-69, 2001 (PubMed).
Zhang, Gerstein: "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome." in: Genomics, Vol. 81, Issue 5, pp. 468-80, 2003 (PubMed).