Breast Carcinoma Amplified Sequence 4 (BCAS4) (N-Term) antibody

Antigen: Breast Carcinoma Amplified Sequence 4 (BCAS4)

Synonyms: CNOL, FLJ20495, MAAB, GAOB1, MGC4973, FLJ20128, DKFZp686O1527, K20D4, rudhira, AU021018, BC028339, 1500019F07Rik, 2610028P08Rik, RGD1560788, MGC63764, zgc:63764, BCAS3, MGC152217

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651915

Additional Information

Alternative name: BCAS4

Gene ID: 55653

UniProt: Q8TDM0, NP_942094.2

Immunogen: This BCAS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from the N-terminal region of human BCAS4.

Isotype: Ig

Description: Other names: Breast carcinoma-amplified sequence 4,BCAS4,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BCAS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-75 amino acids from the N-terminal region of human BCAS4.

Molecular Weight: 22758 DA

Comments:

Background: Breast carcinoma amplified sequence 4 is overexpressed in most breast cancer cell lines. A chromosomal aberration involving this gene may be a cause of breast cancer.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

BCAS4 Antibody (N-term) (ABIN651915) western blot analysis in HL-60 cell line lysates (15ug/lane). This demonstrates the BCAS4 antibody detected the BCAS4 protein (arrow).

BCAS4 Antibody (N-term) (ABIN651915) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCAS4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

BCAS4 Antibody (N-term) (ABIN651915) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Bärlund, Monni, Weaver et al.: "Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer." in: Genes, chromosomes & cancer, Vol. 35, Issue 4, pp. 311-7, 2002 (PubMed).