Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A) (C-Term) antibody

Antigen: Chromatin Assembly Factor 1, Subunit A (p150) (CHAF1A)

Synonyms: CHAF1A, MGC152577, caip150, CAF-Ip150, wu:fd14g10, zgc:153930, LOC398222, xp150, chaf1a-A, CAF1, P150, CAF-1, CAF1B, CAF1P150, MGC71229

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651935

Additional Information

Alternative name: CHAF1A

Gene ID: 10036

UniProt: NP_005474, Q13111

Immunogen: This CHAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-657 amino acids from the C-terminal region of human CHAF1A.

Isotype: Ig

Description: Other names: Chromatin assembly factor 1 subunit A,CHAF1A,CAF1P150, CAF

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CHAF1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-657 amino acids from the C-terminal region of human CHAF1A.

Molecular Weight: 106926 DA

Comments:

Background: Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B, MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

CHAF1A Antibody (C-term) (ABIN651935) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the CHAF1A antibody detected the CHAF1A protein (arrow).

CHAF1A Antibody (C-term) (ABIN651935) western blot analysis in mouse testis tissue lysates (15ug/lane). This demonstrates the CHAF1A antibody detected CHAF1A protein (arrow).

Publications

Product: Verreault, Kaufman, Kobayashi et al.: "Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4." in: Cell, Vol. 87, Issue 1, pp. 95-104, 1996 (PubMed).

Marheineke, Krude: "Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle." in: The Journal of biological chemistry, Vol. 273, Issue 24, pp. 15279-86, 1998 (PubMed).

Shibahara, Stillman: "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin." in: Cell, Vol. 96, Issue 4, pp. 575-85, 1999 (PubMed).

Murzina, Verreault, Laue et al.: "Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins." in: Molecular cell, Vol. 4, Issue 4, pp. 529-40, 1999 (PubMed).