Ras-Related GTP Binding A (RRAGA) (C-Term) antibody

Antigen: Ras-Related GTP Binding A (RRAGA)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651959

Additional Information

Alternative name: RRAGA

Gene ID: 10670

UniProt: Q7L523, NP_006561.1

Immunogen: This RRAGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-312 amino acids from the C-terminal region of human RRAGA.

Isotype: Ig

Description: Other names: Ras-related GTP-binding protein A,RRAGA,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RRAGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-312 amino acids from the C-terminal region of human RRAGA.

Molecular Weight: 36566 DA

Comments:

Background: Involved in the RCC1/Ran-GTPase pathway. RRAGA may play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. biquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

RRAGA Antibody (C-term) (ABIN651959) western blot analysis in Hela cell line lysates (15ug/lane). This demonstrates the RRAGA antibody detected the RRAGA protein (arrow).

RRAGA Antibody (C-term) (ABIN651959) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane). This demonstrates the RRAGA antibody detected the RRAGA protein (arrow).

RRAGA Antibody (C-term) (ABIN651959) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Ashby, Kehoe, Love: "Kallikrein-related peptidase 6 in Alzheimer's disease and vascular dementia." in: Journal of the American Chemical Society, Vol. 1363, Issue Nucleic acids research, pp. 1-10, 2010

Schürmann, Brauers, Massmann et al.: "Cloning of a novel family of mammalian GTP-binding proteins (RagA, RagBs, RagB1) with remote similarity to the Ras-related GTPases." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28982-8, 1996 (PubMed).

Li, Kang, Horwitz: "Interaction of an adenovirus 14.7-kilodalton protein inhibitor of tumor necrosis factor alpha cytolysis with a new member of the GTPase superfamily of signal transducers." in: Journal of virology, Vol. 71, Issue 2, pp. 1576-82, 1997 (PubMed).

Hirose, Nakashima, Sekiguchi et al.: "RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway." in: Journal of cell science, Vol. 111 ( Pt 1), pp. 11-21, 1998 (PubMed).

Sekiguchi, Hirose, Nakashima et al.: "Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7246-57, 2001 (PubMed).

Sekiguchi, Todaka, Wang et al.: "A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D." in: The Journal of biological chemistry, Vol. 279, Issue 9, pp. 8343-50, 2004 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).