WAP Four-Disulfide Core Domain 5 (WFDC5) (N-Term) antibody

Antigen: WAP Four-Disulfide Core Domain 5 (WFDC5)

Synonyms: PRG5, WAP1, dJ211D12.5, Prg5, Wap1, BC019734

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651961

Additional Information

Alternative name: WFDC5

Gene ID: 149708

UniProt: Q8TCV5, NP_663627.1

Immunogen: This WFDC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human WFDC5.

Isotype: Ig

Description: Other names: WAP four-disulfide core domain protein 5,WFDC5,WAP1, PRG5

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This WFDC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human WFDC5.

Molecular Weight: 24238 DA

Comments:

Background: WFDC5 is a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

WFDC5 Antibody (N-term) (ABIN651961) western blot analysis in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the WFDC5 antibody detected the WFDC5 protein (arrow).

WFDC5 Antibody (N-term) (ABIN651961) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WFDC5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

WFDC5 Antibody (N-term) (ABIN651961) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Horikoshi, Cong, Kley et al.: "Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: activation of the human homologue of the Drosophila peroxidasin gene." in: Biochemical and biophysical research communications, Vol. 261, Issue 3, pp. 864-9, 1999 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Clauss, Lilja, Lundwall: "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein." in: The Biochemical journal, Vol. 368, Issue Pt 1, pp. 233-42, 2002 (PubMed).

Mukhopadhyay, Rosen: "The C-terminal domain of the nuclear factor I-B2 isoform is glycosylated and transactivates the WAP gene in the JEG-3 cells." in: Biochemical and biophysical research communications, Vol. 358, Issue 3, pp. 770-6, 2007 (PubMed).