RAN Binding Protein 1 (RANBP1) (C-Term) antibody

Antigen: RAN Binding Protein 1 (RANBP1)

Synonyms: HTF9A, MGC88701, Htf9a, MGC64314, ranbp2, MGC76028, RANBP1, MGC127635, MZN1.4, MZN1_4, RAN BINDING PROTEIN 1, zeh1094, zgc:85649, hm:zeh1094, wu:fa96a09, wu:fj39g07

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652219

Additional Information

Alternative name: RANBP1

Gene ID: 5902

UniProt: P43487

Immunogen: This RANBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-195 amino acids from the C-terminal region of human RANBP1.

Isotype: Ig

Description: Other names: Ran-binding protein 1, RanBP1, Ran-specific GTPase-activating protein,RANBP1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RANBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-195 amino acids from the C-terminal region of human RANBP1.

Molecular Weight: 23310 DA

Comments:

Background: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

RANBP1 Antibody (C-term) (ABIN652219) western blot analysis in Ramos,293,mouse NIH-3T3,MCF-7,HepG2 cell line lysates (35ug/lane).This demonstrates the RANBP1 antibody detected the RANBP1 protein (arrow).

RANBP1 Antibody (C-term) (ABIN652219) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rensen, Roscioli, Tedeschi et al.: "RanBP1 downregulation sensitizes cancer cells to taxol in a caspase-3-dependent manner." in: Oncogene, Vol. 28, Issue 15, pp. 1748-58, 2009 (PubMed).

Lonhienne, Forwood, Marfori et al.: "Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import." in: The Journal of biological chemistry, Vol. 284, Issue 34, pp. 22549-58, 2009 (PubMed).