3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (Center) antibody

Antigen: 3-hydroxyisobutyryl-CoA Hydrolase (HIBCH)

Synonyms: HIBYLCOAH, AI648812, MGC31364, 2610509I15Rik, MGC137758, MGC110824, zgc:110824, T11J7.4, T11J7_4, T11J7.5, T11J7_5

Epitope: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652336

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: HIBCH (Center)

Gene ID: 26275

Swiss-Prot: NP_055177, Q6NVY1

Immunogen: This HIBCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human HIBCH.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial,HIBCH,

Molecular Weight: 43482 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

HIBCH Antibody (Center) (ABIN652336) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the HIBCH antibody detected the HIBCH protein (arrow).

HIBCH Antibody (Center) (ABIN652336) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIBCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

HIBCH Antibody (Center) (ABIN652336) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.