Vitronectin (VTN) (N-Term) antibody

Antigen: Vitronectin (VTN)

Synonyms: VTN, MGC128203, DKFZp470F0511, LOC100228142, vtn, MGC112277, zgc:112277, VN, V75, VNT, Vn, AI256434, Aa1018, MGC124961

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652347

Additional Information

Alternative name: VTN

Gene ID: 7448

UniProt: P04004

Immunogen: This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~101 amino acids from the N-terminal region of human VTN.

Isotype: Ig

Description: Other names: Vitronectin, Serum-spreading factor, S-protein, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B , VTN

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~101 amino acids from the N-terminal region of human VTN.

Molecular Weight: 54,306 DA

Comments:

Background: VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of VTN Antibody (N-term) (ABIN652347) in NCI-H460 cell line lysates (35ug/lane). VTN (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with VTN Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

VTN Antibody (N-term) (ABIN652347) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Seiffert, Loskutoff: "Evidence that type 1 plasminogen activator inhibitor binds to the somatomedin B domain of vitronectin." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2824-30, 1991 (PubMed).

Jenne, Stanley: "Molecular cloning of S-protein, a link between complement, coagulation and cell-substrate adhesion." in: The EMBO journal, Vol. 4, Issue 12, pp. 3153-7, 1986 (PubMed).

Sigurdardottir, Wiman: "Identification of a PAI-1 binding site in vitronectin." in: Biochimica et biophysica acta, Vol. 1208, Issue 1, pp. 104-10, 1994 (PubMed).