Anaplastic Lymphoma Receptor tyrosine Kinase (ALK) (Center) antibody

Antigen: Anaplastic Lymphoma Receptor tyrosine Kinase (ALK)

Synonyms: CD246, NBLST3, TFG/ALK, Tcrz, ALK

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652362

Additional Information

Alternative name: ALK

Gene ID: 238

UniProt: Q9UM73

Immunogen: This ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644~673 amino acids from the center region of human ALK.

Isotype: Ig

Description: Other names: Anaplastic lymphoma kinase, CD246 antigen

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644~673 amino acids from the center region of human ALK.

Molecular Weight: 176417 DA

Comments:

Background: ALK, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t(2,5)(p23,q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody (Center) (ABIN652362), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of ALK(Center)(arrow) using rabbit polyclonal ALK(Center) Antibody (ABIN652362).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK (Center) gene (Lane 2) (Origene Technologies).

Publications

Product: Morris, Kirstein, Valentine et al.: "Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma." in: Science (New York, N.Y.), Vol. 263, Issue 5151, pp. 1281-4, 1994 (PubMed).

Iwahara, Fujimoto, Wen et al.: "Molecular characterization of ALK, a receptor tyrosine kinase expressed specifically in the nervous system." in: Oncogene, Vol. 14, Issue 4, pp. 439-49, 1997 (PubMed).

Morris, Naeve, Mathew et al.: "ALK, the chromosome 2 gene locus altered by the t(2;5) in non-Hodgkin's lymphoma, encodes a novel neural receptor tyrosine kinase that is highly related to leukocyte tyrosine kinase (LTK)" in: Oncogene, Vol. 14, Issue 18, pp. 2175-88, 1997 (PubMed).