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V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody

Antigen

V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF)
Synonyms BRAF1, RAFB1, B-RAF1, FLJ95109, MGC126806, MGC138284, RMIL, C-RMIL, B-Raf, braf1, rafb1, b-raf1, BRAF
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), ELISA
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN652396
Quantity 0.1 mg  (0.25 mg/ml)
Price 280.50 $   Plus shipping costs $45.00
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anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody

anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) antibody (2)

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name BRAF
Gene ID 673
UniProt P15056
Immunogen This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.
Isotype Ig
Description Other names: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Characteristics Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity This BRAF antibody is generated from rabbits immunized with BRAF recombinant protein.
Molecular Weight 84490 DA
Comments

Background: BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Application Details
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Application Notes The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Flow cytometry ~~ 1:10~50
Concentration 0.25 mg/ml
Purification Purified
Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
Research Area Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology
Restrictions For Research Use only

Publications
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Product Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).

Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).

Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF)", type "Antibodies"
Hosts Rabbit (33), Mouse (26), Chicken (1)
Reactivities Human (56), Monkey (6), Dog (Canine) (3), Mouse (Murine) (2), Rat (Rattus) (1)
Applications Western Blotting (WB) (46), ELISA (39), Immunohistochemistry (IHC) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunofluorescence (IF) (7), Dot Blot (Dot) (5), Flow Cytometry (FACS) (3), Functional Studies (Func) (1)
Epitopes C-Term (2), Internal Region (2), Ser578 (2), Thr400 (2), pSer445 (2), pSer601 (2), pThr400 (2), pThr439 (2), pThr598 (2), AA 10-22 (1), AA 405-524 (1), AA 754-765 (1), Center, Ser364 (1), N-Term (1), Ser364 (1), Ser428 (1), Ser445 (1), Ser601 (1), Ser728 (1), Thr372 (1), Thr439 (1), Thr598 (1), pThr598, pSer601 (1)